Methods and compositions for treating abnormal cell growth...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/12 (2006.01) A61K 31/70 (2006.01) A61K 38/17 (2006.01) A61K 48/00 (2006.01) C07K 14/82 (2006.01) C07K 16/32 (2006.01) C12N 5/10 (2006.01) G01N 33/574 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2287540

Methods and compositions are described that affect the GTPase activity of members of the Ras superfamily, preferably Rac, such compositions include guanine nucleotide exchange factors that modulate the GTPase activity, preferably in the presence of GEF enhancers, exemplary guanine nucleotide exchange factors being Rac-GEF and Tiam-1, which are encoded by certain nucleic acid sequences that are herein described, along with uses for the guanine nucleotide exchange factors and the nucleic acid sequences including screening for ligands which recognize Rac-GEF, regulators of Rac-GEF activity, and methods of treating pathological conditions associated or related to a Ras superfamily GTPase, including Rac.

L'invention concerne des procédés et des compositions ayant un impact sur l'activité GTPase de membres de la superfamille Ras, de préférence la Rac. Ces compositions comprennent des facteurs d'échange des nucléotides à base de guanine, qui modulent l'activité GTPase, de préférence en présence d'activateurs GEF, les facteurs d'échange cités en exemple étant Rac-GEF et Tiam-1, qui sont codés par certaines séquences d'acides nucléiques décrites ici. L'invention concerne également des modes d'utilisation de ces facteurs d'échange et des séquences d'acides nucléiques, notamment le titrage de ligands reconnaissant Rac-GEF, des régulateurs de l'activité Rac-GEF, et des méthodes permettant de traiter des états pathologiques associés ou liés à une GTPase de la superfamille Ras, y compris Rac.

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