Methods and compositions for treating disease

A - Human Necessities – 61 – K

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Details

A61K 48/00 (2006.01) A61K 31/711 (2006.01) C12N 15/00 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2658836

The present invention relates to methods and compositions for treating a subject comprising destroying diseased cells in the subject. The methods comprise obtaining a population of cells from a subject and determining the activity of at least one disease marker gene within the population of the obtained cells. A polynucleotide molecule that encodes a polypeptide that is lethal to the cells is then introduced into the cells, where the expression of the lethal polypeptide is controlled by the promoter of at least one of the disease marker genes previously identified. After introduction of the polynucleotide, the cells are treated with conditions to induce expression of the lethal polypeptide to destroy the cells that are expressing the disease marker gene(s). After destruction of the diseased cells, the remaining live cells, which did not express the lethal polypeptide to an extent necessary to kill the cells, are separated from the dead cells, and the live cells are restored to the subject.

La présente invention concerne des méthodes et des compositions de traitement d'un sujet comprenant la destruction des cellules malades chez le sujet. Ces méthodes consistent à prélever une population de cellules sur un sujet et à déterminer l'activité d'au moins un gène marqueur de maladie dans la population de cellules prélevées. Une molécule de polynucléotide codant un polypeptide létal pour les cellules est ensuite introduite dans les cellules, l'expression du polypeptide létal étant régulée par le promoteur d'au moins un des gènes marqueurs de maladie précédemment identifiés. Après l'introduction du polynucléotide, les cellules sont traitées de façon que l'expression du polypeptide létal soit induite afin que les cellules exprimant le ou les gènes marqueurs de maladie soient détruites. Après la destruction des cellules malades, les cellules vivantes restantes, qui n'exprimaient pas le polypeptide létal d'une manière suffisante pour tuer les cellules, sont séparées des cellules mortes puis restituées au sujet.

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