Methods and compositions for use in modulating expression of...

C - Chemistry – Metallurgy – 12 – Q

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Details

C12Q 1/68 (2006.01) A61K 31/07 (2006.01) A61K 31/195 (2006.01) A61K 31/196 (2006.01) A61K 31/335 (2006.01) A61K 31/357 (2006.01) A61K 45/06 (2006.01) C12N 9/64 (2006.01)

Patent

CA 2288272

The present invention relates to methods for identifying and selecting compositions useful in differentially modulating the expression of two or more mammalian genes, particularly matrix metalloproteinase (MMP) genes such as those encoding interstitial collagenase (and other genes comprising an APl- binding site) and stromelysin-3 (and other genes comprising a retinoic acid response element (RARE)). In addition, the invention relates to methods of treating a mammal (such as a human) suffering from or predisposed to a physical disorder, using pharmaceutical compositions comprising the compositions identified or selected by the above-described methods. The methods of the present invention are useful in treating a variety of physical disorders in mammals including cancers (particularly carcinomas), inflammatory disorders, fibrotic disorders, ocular disorders and osteoporosis.

L'invention concerne des procédés d'identification et de sélection de compositions utiles dans la modulation différentielle de l'expression d'au moins deux gènes mammaliens, notamment des gènes de métalloprotéinases matricielles tels que ceux codant la collagénase interstitielle (et d'autres gènes comprenant un site de liaison d'AP-1) et la stromélysine-3 (et d'autres gènes comprenant un élément de réponse à l'acide rétinoïque (RARE)). De plus, l'invention se rapporte à des procédés de traitement d'un mammifère (tel qu'un humain) souffrant d'un trouble physique ou prédisposé à celui-ci, au moyen de compositions pharmaceutiques comprenant les compositions identifiées ou sélectionnées par les procédés susmentionnés. Les procédés de l'invention sont utiles dans le traitement d'un grand nombre de troubles physiques chez les mammifères, dont les cancers (notamment les carcinomes), les troubles inflammatoires, les troubles fibreux, les troubles oculaires et l'ostéoporose.

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