Methods and compositions related to immunizing against...

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/085 (2006.01) A61K 39/395 (2006.01) A61P 31/04 (2006.01) C07K 16/12 (2006.01)

Patent

CA 2697538

Embodiments of the invention include methods and compositions useful in a vaccination strategy capable of neutralizing HIa to provide immunoprotection against S. aureus pneumonia. In certain aspects the invention includes a HIa with reduced toxicity, represented by a recombinant mutant form of HIa (HlaH35L) in which histidine 35 is converted to leucine, which can be used to abrogate the productive assembly of the toxin and protect a subject from staphylococcal pneumonia.

Selon des modes de réalisation de l'invention, des procédés et des compositions sont utiles dans une stratégie de vaccination capable de neutraliser le HIa pour fournir une immunoprotection contre une pneumonie par S. aureus. Sous certains aspects, l'invention comprend un HIa ayant une toxicité réduite, représenté par une forme mutante recombinante du HIa (HIaH35L) dans laquelle l'histidine 35 est convertie en leucine, qui peut être utilisée pour abroger l'assemblage productif de la toxine et pour protéger un sujet d'une pneumonie staphylococcique.

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