C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01)
Patent
CA 2401495
Genetic constructs and methods are disclosed for the production, maintenance and control of transgenes in transgenic eukaryotic organisms that undergo meiosis in which pollen or sperm can be outcrossed; this includes: transgenic animals, plant cells, plant tissues and whole plants. More specifically, this invention relates to the control of transgene transmission by male and/or female gametes or gametophytes using a gametophytic sterility trait (GST). The genetic constructs and methodologies of the present invention provide the ability to control the undesired spread of transgenes. In addition, this invention also provides the tools and methodologies to enrich a plant or other eukaryotic genome for dispersed and/or stable transposition events.
L'invention concerne des constructions génétiques et des procédés de production, d'entretien et de régulation de transgènes dans des organismes eucaryotes transgéniques soumis à la méiose dans laquelle le pollen ou le sperme peut être croisé, à savoir: des animaux transgéniques, des cellules végétales, des tissus végétaux et des plantes entières. Plus particulièrement, cette invention concerne la régulation de la transmission de transgènes par des gamètes ou des gamétophytes mâles et/ou femelles au moyen du caractère de stérilité gamétophytique (GST). Les constructions génétiques et les méthodologies de l'invention permettent de réguler la dispersion non désirée de transgènes. En outre, l'invention concerne des outils et des méthodologies d'enrichissement d'une plante et d'autres génomes eucaryotes pour des transpositions dispersées et/ou stables.
Dellaporta Stephen L.
Moreno Maria A.
Gowling Lafleur Henderson Llp
Yale University
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