Methods and constructs for expressing polypeptide multimers...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/79 (2006.01) A61K 38/00 (2006.01) A61K 39/395 (2006.01) A61K 48/00 (2006.01) C07K 16/00 (2006.01) C12N 15/81 (2006.01) C12N 15/86 (2006.01) C12P 21/02 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2559280

The invention provides a method of producing multiple polypeptides, such as antibodies or antibody fragments, in a eukaryotic cell using a single expression vector. The expression vector is engineered to comprise two or more expression cassettes under the control of a single promoter wherein the expression cassettes have splice sites which allow for their alternative splicing and expression as two or more independent gene products at a desired ratio. Use of the vector for the efficient expression of recombinant antibodies in eukaryotic host cells is disclosed as well as the use of such antibodies in diagnostic and therapeutic applications.

L'invention concerne une méthode qui permet de produire des polypeptides multiples, tels que des anticorps ou des fragments d'anticorps, dans une cellule eucaryote au moyen d'un seul vecteur d'expression. Le vecteur d'expression est modifié de façon qu'il comprenne au moins deux cassettes d'expression contrôlées par un seul promoteur, ces cassettes d'expression comprenant des sites d'épissage qui permettent un épissage alternatif de ces dernières et leur expression sous forme d'au moins deux produits géniques indépendants à un ratio souhaité. L'invention concerne l'utilisation dudit vecteur pour exprimer efficacement des anticorps recombinés dans les cellules hôtes eucaryotes ainsi que l'utilisation de ces anticorps dans des applications diagnostiques et thérapeutiques.

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