Methods and devices for detection and identification of...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2637974

The invention relates to methods and devices used in the sequencing, separation, detection, and identification of objects and biological molecules. In preferred embodiments, the invention relates to a DNA sequencing system based on cyclic sequencing by synthesis which is performed on beads in three- dimensional vessels and detected using monolithic multicapillary arrays. In other embodiments, the invention relates to a bead comprising two or more luminescent labels coupled to a nucleic acid sequence. In further embodiments, said luminescent labels are quantum dots.

L~invention concerne des procédés et dispositifs utilisés pour le séquençage, la séparation, la détection et l~identification d~objets et de molécules biologiques. Dans des modes de réalisation préférés, l~invention concerne un système de séquençage d~ADN s~appuyant sur un séquençage cyclique par synthèse qui est mis en AEuvre sur des billes dans des modèles de vaisseaux en trois dimensions et détecté en utilisant des réseaux multicapillaires monolithiques. Dans d~autres modes de réalisation, l~invention concerne une bille comprenant au moins deux marqueurs luminescents couplés à une séquence d~acide nucléique. Dans d~autres modes de réalisation, lesdits marqueurs luminescents sont des points quantiques.

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