Methods and devices for dispersing somatic plant embryos

A - Human Necessities – 01 – H

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A01H 4/00 (2006.01) B01L 3/00 (2006.01)

Patent

CA 2721070

Methods and devices for dispersion of clusters of somatic plant embryos suspended in a liquid are disclosed. The methods comprise i) subjecting the clusters of embryos to fluid dynamics forces causing axially extensional strain and radially compressional strain and ii) subjecting the clusters of embryos to fluid dynamics forces causing axially compressional strain and radially extensional strain fluid dynamics and iii) repeating said steps in sequence until the individual embryos are separated from each other. The devices may comprise a flow channel including at least one constriction, such that clusters of embryos flowing through the flow channel are first subjected to axially extensional strain and radially compressional strain, and then to axially compressional strain and radially extensional strain from fluid dynamics forces.

La présente invention concerne des procédés et des dispositifs de dispersion densembles dembryons somatiques de plantes mis en suspension dans un liquide. Lesdits procédés comprennent les étapes suivantes : i) soumission des ensembles dembryons à des forces de dynamique des fluides provoquant une déformation de traction axiale et une déformation de compression radiale; et ii) soumission des ensembles dembryons à des forces de dynamique des fluides provoquant une dynamique de fluide de déformation de compression axiale et de déformation de traction radiale; et iii) répétition de ces étapes en séquence jusquà ce que les embryons individuels soient séparés les uns des autres. Ces dispositifs peuvent comprendre un canal découlement incluant au moins un resserrement, de telle sorte que les ensembles dembryons sécoulant à travers le canal découlement soient tout dabord soumis à une déformation de traction axiale et à une déformation de compression radiale, puis à une déformation de compression axiale et à une déformation de traction radiale provenant des forces de la dynamique des fluides.

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