Methods and devices for producing biomolecules

Details Methods and devices for producing biomolecules Methods and devices for producing biomolecules

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) B01D 1/24 (2006.01) B03D 1/18 (2006.01)

Patent

CA 2714151

A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO2 bubbles on the precipitate floe. The CO2 is released from a carbonate salt during or after neutralization (acidification). The method of the invention is preferably carried out in an automated continuous mode applying devices for lysis and neutralization and a novel device for completely continuous clarification (separation of floes and clarified lysate).

Linvention concerne un procédé et un dispositif à échelle variable pour produire une biomolécule, en particulier un ADN plasmidique de qualité pharmaceutique. Le procédé comprend les étapes de lyse alcaline, de neutralisation et de clarification et peut être encore prolongé. Linvention concerne également un procédé de flottation perfectionné pour séparer le lysat et le précipité. Ce procédé est basé sur la fixation de bulles de CO2 sur le floc précipité. Le CO2 est dégagé à partir dun sel de carbonate au cours ou après la neutralisation (acidification). Le procédé de linvention est de préférence mis en uvre dans un mode continu automatisé appliquant des dispositifs pour la lyse et la neutralisation et un nouveau dispositif pour la clarification complètement continue (séparation des flocs et du lysat clarifié).

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