Methods and devices for the long-term culture of...

Details Methods and devices for the long-term culture of... Methods and devices for the long-term culture of...

C - Chemistry, Metallurgy

12

N

C12N 5/078 (2010.01) C12N 5/0789 (2010.01) A61K 35/14 (2006.01) A61L 27/38 (2006.01) C12M 3/00 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2304650

The invention pertains to methods and devices for the long term, in vitro culture of hematopoietic progenitor cells in the absence of exogenously added hematopoietic growth factors, improved methods for the introduction of foreign genetic material into cells of hematopoietic origin, and to apparatus for performing these methods. The hematopoietic progenitor cells are cultured on a three-dimensional porous biomaterial. The three-dimensional porous biomaterial enhances hematopoietic progenitor cell survival and leads to an expansion of progenitor cell numbers and/or functionality, while maintaining progenitor cell pluripotency in the absence of exogenous growth factors. In addition, the three-dimensional porous biomaterial supports high level transduction on cells cultured upon such environment.

L'invention concerne des procédés et des dispositifs de vitroculture à long terme de cellules souches hématopoïétiques en l'absence de facteurs de croissance hématopoïétiques ajoutés par voie exogène, des procédés améliorés d'introduction de matériel génétique étranger dans des cellules d'origine hématopoïétique, ainsi qu'un appareil de mise en application de ces procédés. Les cellules souches hématopoïétiques sont mises en culture sur un biomatériau poreux tridimensionnel. Le biomatériau poreux tridimensionnel augmente le taux de survie des cellules souches hématopoïétiques et permet une expansion des nombres et/ou de la fonctionnalité des cellules souches, tout en maintenant l'activité multipotente des cellules souches en l'absence de facteurs de croissance exogènes. De plus, le biomatériau poreux tridimensionnel permet une transduction de haut niveau dans des cellules mises en culture sur cet environnement.

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