Methods and devices for the quantitative detection of...

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G01N 33/48 (2006.01) C12Q 1/68 (2006.01) G01J 3/00 (2006.01) G01N 33/50 (2006.01) G01N 33/543 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2428011

The invention provides for the detection and quantification of PSMA, PSMA', and other prostatic markers in serum samples as well as in other types of samples for use in differentiating prostate cancer, benign prostatic hyperplasia, and negative diagnoses. The diagnostic detection of nucleic acids, such as mRNAs, which encode prostatic markers in cell lysates and other sample sources is also provided. In addition to the multiplexed detection/quantification of these protein- and nucleic acid-based markers, the invention also includes biochips, kits and integrated systems.

L'invention concerne la détection et la quantification du PSMA, du PSMA', et d'autres marqueurs prostatiques dans des échantillons de sérum ainsi que dans d'autres types d'échantillons afin de permettre la différentiation entre un cancer de la prostate, les hyperplasies bénignes de la prostate et les diagnostics négatifs. L'invention concerne également la détection diagnostique d'acides nucléiques tels que les ARNm, qui codent pour des marqueurs prostatiques dans les lysats de cellules et dans d'autres sources d'échantillons. Outre la détection/quantification combinée de ces marqueurs à base de protéines et d'acides nucléiques, l'invention concerne des biopuces, des trousses de matériel et des système intégrés.

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