Methods and gel compositions for encapsulating living cells...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/00 (2006.01) A61K 9/16 (2006.01) C08G 18/10 (2006.01) C08G 65/333 (2006.01) C08G 65/334 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2443060

A method for encapsulating biologics within a hydrogel by using an aqueous solution of an isocyanate-functional hydrogel prepolymer which is mixed with an amount of biologics and an aqueous solution containing a dithiol crosslinking agent under physiological pH conditions. An additional bidentate crosslinking agent may be included. The product of such method may be a bioreactor or an assay device having a plurality of different biologics encapsulated at predetermined locations in a substrate.

L'invention concerne une méthode d'encapsulation de substances biologiques à l'intérieur d'un hydrogel consistant à utiliser une solution aqueuse d'un prépolymère d'hydrogel à fonctionnalité isocyanate lequel est mélangé avec une dose de substances biologiques et une solution aqueuse contenant un agent réticulant dithiol dans des conditions de pH physiologique. Un autre agent réticulant bidenté peut aussi être prévu. Le produit de cette méthode peut être un bioréacteur ou un dispositif de dosage ayant une pluralité de substances biologiques différentes encapsulées en des emplacements prédéterminés dans un substrat.

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