Methods and kits for analyzing polynucleotide sequences

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2557177

The present invention features methods for analyzing a sequence of a target polynucleotide by detecting incorporation of a nucleotide into its complementary strand, where the polynucleotides may be bound at high density and at single molecule resolution. The invention also features labeling moieties and blocking moieties, which facilitate chain tern nation or choking. Certain aspects provide for temporal detection of the incorporations; some allow for asynchronous analysis of a plurality of target polynucleotides and the use of short sequencing cycles. Surface chemistry aspects of the sequencing methods are also provided. The method may also be used in kits, said kits designed to carry out and facilitate the methods provided herein.

La présente invention concerne des procédés pour analyser une séquence d'un polynucléotide cible. Ces procédés consistent à détecter l'introduction d'un nucléotide dans son brin complémentaire, le polynucléotide pouvant être lié à une haute densité et à une résolution d'une seule molécule. La présente invention concerne également des groupes de marquage et des groupes de blocage qui facilitent la terminaison ou le blocage de chaîne. Certains aspects de cette invention concernent la détection temporelle des introductions, certains permettent une analyse asynchrone d'une pluralité de polynucléotides cibles et l'utilisation de cycles de séquençage courts. Cette invention a aussi trait à des aspects de chimie de surface des procédés de séquençage. Le procédé peut également être utilisé dans des kits qui sont conçus pour mettre en oeuvre et faciliter lesdits procédés.

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