C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/28 (2006.01)
Patent
CA 2576870
The present invention provides a method for assaying myeloperoxidase activity. In the assay, a sample containing myeloperoxidase or suspected of containing myeloperoxidase is contacted with substrate including serine, hydrogen peroxide, and a halide. If myeloperoxidase is present in the sample, serine is converted into glycolaldehyde, which is further converted into glycolate by a glycoaldehyde converting enzyme (e.g. glycolaldehyde dehydrogenase) (1.2.1.21). The method then utilizes a cycling reaction system between glycolate and glyoxylate to generate a detectable signal that corresponds to the myeloperoxidase activity. Kits for assaying myeloperoxidases based on the same principle are also provided.
L'invention porte sur un procédé d'essai de l'activité de la myéloperoxidase selon lequel un échantillon contenant de la myéloperoxidase ou susceptible d'en contenir est mis en contact avec un substrat comportant de la sérine, du peroxyde d'hydrogène et un halogénure. Si la myéloperoxidase est présente dans l'échantillon, la sérine est convertie en glycolaldéhyde qui est ensuite convertie en glycolate par une enzyme de conversion de la glycoaldéhyde (par exemple la glycoaldéhyde déshydrogénase) (1.2.1.21). Le procédé utilise un système de réaction de cyclage entre le glycolate et le glyoxylate pour produire un signal détectable correspondant à l'activité de la myéloperoxidase. L'invention porte également sur des trousses d'essai de la myéloperoxidase basées sur le même principe.
Dou Chao
Yuan Chong-Sheng
Fetherstonhaugh & Co.
General Atomics
LandOfFree
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