Methods and kits for determining pre-amplification levels of...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2223241

Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducibility. Also described are useful methods for extending the dynamic range of transcription-based amplification systems.

Procédés et trousses permettant de mettre en rapport les quantités initiales d'acides nucléiques cibles présentes dans un échantillon avec des produits d'amplification à cible spécifique. On a découvert que le système d'amplification à médiation de transcription est à même de produire un rapport quantitatif entre une entrée cible et une sortie spécifique à une cible. En outre, l'invention se rapporte à des procédés permettant de modules soigneusement ce rapport, avec pour résultat inattendu un taux élevé de reproductibilité. L'invention concerne également des procédés qui permettent d'étendre la plage dynamique des systèmes d'amplification à base de transcription.

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