Methods and kits for dual enzymatic assays whereby light is...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/66 (2006.01) C09K 11/00 (2006.01) G01N 21/76 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2515217

The present invention relates to single and dual reporter luminescence assays utilizing reagents to quench an optical, e.g., an enzyme-mediated luminescence, reaction. In one embodiment of the invention, a reagent is added to an assay which selectively quenches a first enzyme-mediated luminescence reaction without affecting a subsequent distinct enzyme-mediated luminescent reaction(s). An assay kit containing one or more selective quench reagents, and compositions comprising the quench reagent(s), are also provided.

L'invention a trait à des dosages de luminescence de rapporteurs simples et doubles, qui font appel à des réactifs permettant de désactiver une réaction optique, par exemple une réaction de luminescence provoquée par des enzymes. Dans un mode de réalisation de la présente invention, un réactif est ajouté à un dosage qui désactive sélectivement une première réaction de luminescence provoquée par des enzymes, sans avoir d'effet sur une ou plusieurs réactions luminescentes provoquées par des enzymes distinctes ultérieures. L'invention concerne également un kit de dosage contenant un ou plusieurs réactifs de désactivation sélectifs, et des compositions contenant le ou les réactifs de désactivation.

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