Methods and kits for testing the efficacy of therapeutic...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/02 (2006.01) G01N 33/15 (2006.01)

Patent

CA 2691044

Three-dimensional physiological matrices, methods, apparatus and kits lor the expedited design, testing and evaluation of oncological remedies are provided. Key aspects of the inventions include matrices, and especially gel matrices, comprising one or more physiological fibers, which are adapted and arranged to provide conditions which permit behaviors, such as the movement of cells away from the margins of samples of target tissue through the matrix, to be evaluated in a manner that produces data useful for evaluating the oncological status and characteristics of the cells. In a further key aspect, the invention permits the in vitro testing and analyses of one or more conventional, experimental or theoretical therapies with respect to specific target tissues or cells. Among such therapies are therapeutic compounds and combinations thereof, radiation therapies, combinations of therapeutic compounds and radiation and numerous other possible therapies.

La présente invention concerne des matrices physiologiques tridimensionnelles, des procédés, un appareil et des trousses permettant la conception, l'analyse et l'évaluation accélérées de remèdes oncologiques. Les aspects principaux selon l'invention incluent des matrices, et particulièrement des matrices de gel, renfermant une ou plusieurs fibres physiologiques, qui sont conçues et disposées pour produire des conditions permettant d'évaluer des événements, tels que le déplacement de cellules loin des limites d'échantillons de tissu cible au travers de la matrice, selon un mode qui produit des données utiles à l'évaluation du statut oncologique et des caractéristiques des cellules. Dans un autre aspect important, l'invention concerne des tests et des analyses in vitro d'une ou de plusieurs thérapies classiques, expérimentales ou théoriques relatives à des tissus ou des cellules cibles spécifiques. Des composés thérapeutiques et leurs combinaisons, des thérapies par irradiation, des combinaisons de composés thérapeutiques et de radiations et de nombreuses autres thérapies possibles font partie de telles thérapies.

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