Methods and materials for making and using transgenic...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) A01H 1/00 (2006.01) A01H 3/00 (2006.01) A01H 4/00 (2006.01) A01H 5/00 (2006.01) C07K 14/79 (2006.01) C12N 9/00 (2006.01) C12N 9/02 (2006.01) C12N 9/10 (2006.01) C12N 15/09 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2285676

The invention provides isolated and at least partially-purified dicamba- degrading enzymes, isolated DNA molecules coding for dicamba-degrading enzymes, DNA constructs coding for dicamba-degrading enzymes, transgenic host cells comprising DNA coding for dicamba-degrading enzymes, and transgenic plants and plant parts comprising one or more cells comprising DNA coding for dicamba-degrading enzymes. Expression of the dicamba-degrading enzymes results in the production of dicamba-degrading organisms, including dicamba-tolerant plants. The invention further provides a method of controlling weeds in a field containing the transgenic dicamba-tolerant plants of the invention and a method of decontaminating a material containing dicamba comprising applying an effective amount of a transgenic microorganism or dicamba-degrading enzyme of the invention to the material. Finally, the invention provides a method of selecting transformed plants and plant cells based on dicamba tolerance and a method of selecting or screening transformed host cells, intact organisms and parts of organisms based on the fluorescence of 3,6-dichlorosalicylic acid produced as a result of dicamba degradation.

L'invention concerne des enzymes isolées et au moins partiellement purifiées dégradant dicamba, des molécules d'ADN isolées qui codent pour des enzymes dégradant dicamba, des constructions d'ADN qui codent pour des enzymes dégradant dicamba, des cellules hôtes transgéniques comprenant un ADN qui code pour des enzymes dégradant dicamba, et des plantes transgéniques et éléments de plante qui comprennent une ou plusieurs cellules incluant un ADN qui code pour des enzymes dégradant dicamba. L'expression des enzymes dégradant dicamba donne lieu à la production d'organismes dégradant dicamba, y compris des plantes tolérant dicamba. L'invention concerne en outre une méthode qui permet de lutter contre les mauvaises herbes dans un champ contenant lesdites plantes tolérant dicamba, et une méthode pour décontaminer un matériel contenant dicamba, qui consiste à appliquer sur ledit matériel une quantité efficace d'un micro-organisme transgénique ou d'une enzyme dégradant dicamba. L'invention concerne enfin une méthode de sélection de plantes modifiées et de cellules végétales basée sur la tolérance de dicamba et une méthode de sélection et de criblage de cellules hôtes modifiées, d'organismes et d'éléments d'organismes intacts sur la base de la fluorescence de 3,6-acide dichlorosalicylique résultant de la dégradation de dicamba.

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