Methods and materials for optimization of electronic...

Details Methods and materials for optimization of electronic... Methods and materials for optimization of electronic...

C - Chemistry, Metallurgy

07

K

C07K 1/26 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2579124

Methods for the transport and hybridization of a nucleic acid on an electrode device by providing a low conductivity buffer with a reducing agent to the device. The low conductivity buffer may also contain a zwitterion. A current and voltage is applied to a location of the device to effect electrophoretic transportation of the nucleic acid towards the location. The nucleic acid is then hybridized to a nucleic probed located at the location. The reducing agent in the low conductivity buffer may also be acting as a chaotropic agent.

L'invention concerne des procédés pour le transport et l'hybridation d'un acide nucléique sur un dispositif d'électrode qui consistent à équiper le dispositif d'un tampon à faible conductivité pourvu d'un agent réducteur. Le tampon à faible conductivité peut également contenir un zwittérion. Un courant et une tension sont appliqués sur un emplacement du dispositif afin d'effectuer le transport électrophorétique de l'acide nucléique vers ledit emplacement. L'acide nucléique est hybridé dans une sonde nucléique située sur ledit emplacement. L'agent réducteur situé dans le tampon à faible conductivité peut également agir comme agent chaotropique.

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