Methods and materials for phe a feedback inhibition resistance

Details Methods and materials for phe a feedback inhibition resistance Methods and materials for phe a feedback inhibition resistance

C - Chemistry, Metallurgy

12

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195/1.235

C12N 15/61 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/60 (2006.01) C12P 13/22 (2006.01)

Patent

CA 2025491

Disclosed are DNA sequences encoding deletion, substitution and/or addition analogs of the E. coli enzyme, chorismate mutase/prephenate dehydratase (CMPD). Preferred expression products include [des-Gln307, des-Ala308, des-Gly309, des-Ala310]CMPD; [Leu306]CMPD; [des-Thr304, Lys305, des-Gln306]CMPD; and [Cys309]CMPD display enzymatic activity of the wild type enzyme but are more resistant to inhibition in the presence of phenylalanine.

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