C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/34 (2006.01) A01H 5/00 (2006.01) C07K 14/01 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2229168
This invention comprises a mutant plant virus gene which confers resistance on tobacco and tomato plants against tobacco mosaic tobamovirus and tomato mottle geminivirus infections and infection by other related geminiviruses. A gene was initially isolated from the known BC1 gene, between nucleotides 1278 and 2311 of the B component of tomato mottle geminivirus. Upon subcloning of this DNA fragment into an appropriate expression vector and transformation of the gene into tobacco plants, a truncated gene product was produced which confers resistance against viral infection to the recombinant plant in which it is expressed.
Cette invention concerne un gène mutant de virus végétal qui confère au tabac et aux tomates une résistance au virus de la mosaïque du tabac et aux infections dues au géminivirus de la marbrure de la tomate, ainsi qu'aux infections dues à d'autres géminivirus apparentés. On a d'abord isolé un gène du gène BC1 connu, entre les nucléotides 1278 et 2311 du composant B du géminivirus de la marbrure de la tomate. Lors du sous-clonage de ce fragment d'ADN en un vecteur d'expression approprié et de la transformation du gène dans le tabac, on obtient un produit génique tronqué qui confère à la plante de recombinaison qui est exprimée une résistance aux infections virales.
Abouzid Ahmed
Duan Yong Ping
Hiebert Ernest
Polston Jane E.
Powell Charles A.
Sim & Mcburney
University Of Florida
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