Methods and materials for the production of lactic acid in...

C - Chemistry – Metallurgy – 12 – P

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C12P 7/56 (2006.01) C07H 21/04 (2006.01) C12N 1/16 (2006.01) C12N 9/04 (2006.01) C12N 15/81 (2006.01)

Patent

CA 2488196

Recombinant yeast are provided having, in one aspect, multiple exogenous LDH genes integrated into genome, while leaving native PDC genes intact. In a second aspect, recombinant yeast are provided having an exogenous LDH gene integrated into its genome at the locus of a native PDC gene, with deletion of the native PDC gene. The recombinant yeast are useful in fermentation process for producing lactic acid.

La présente invention concerne des levures recombinées qui, dans un de leurs aspects, comprennent des gènes LDH exogènes multiples intégrés dans le génome, alors que les gènes PDC natifs restent intacts. L'invention concerne, dans un second aspect, des levures recombinées qui comprennent un gène LDH exogène intégré dans le génome sur le locus d'un gène PDC natif, avec délétion du gène PDC natif. Les levures recombinées de l'invention sont utilisées dans des processus de fermentation destinés à la production d'acide lactique.

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