Methods and materials for using chemical compounds as a tool...

Details Methods and materials for using chemical compounds as a tool... Methods and materials for using chemical compounds as a tool...

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) C07H 1/08 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2501056

The present invention relates to methods for isolating and storing, nucleic acid from a sample containing nucleic acid, such as a cell sample or cell lysate. The nucleic acid is isolated on a solid phase medium, which is then dried, and which can be stored efficiently, such as at room temperature, in columns, tubes, and microwell plates having a wide variety of filters and other solid phase media, for extended periods of time, including days, weeks, and months. The invention provides methods for isolating and storing nucleic acid from a sample by applying the sample to a solid phase medium, retaining the cells, lysing the cellular retentate, drying the medium and retaining the nucleic acid, storing the nucleic acid for extended periods of time at room temperature and humidity, and optionally eluting the nucleic acid. The invention provides methods for storing nucleic acid-containing samples on a wide range of solid phase media in many types of tubes, columns, or multiwell plates, many of which are commercial available.

L'invention concerne des méthodes permettant d'isoler et de stocker de l'acide nucléique provenant d'un échantillon contenant de l'acide nucléique, notamment un échantillon cellulaire ou un lysat cellulaire. L'acide nucléique est isolé sur un support en phase solide, est séché, et peut être efficacement stocké, notamment à température ambiante, dans des colonnes, des tubes, et des plaques de microcupules présentant une grande variété de filtres et d'autres supports en phase solide, pendant des périodes prolongées, notamment des jours, des semaines et des mois. L'invention concerne des méthodes permettant d'isoler et de stocker de l'acide nucléique provenant d'un échantillon, consistant à appliquer l'échantillon sur un support en phase solide, à retenir les cellules, à lyser le retentat cellulaire, à sécher le support et à retenir l'acide nucléique, à stocker l'acide nucléique pendant des périodes prolongées, à température et à humidité ambiantes, et éventuellement, à éluer l'acide nucléique. L'invention concerne des méthodes pour stocker des échantillons contenant de l'acide nucléique sur une grande variété de supports en phase solide, dans plusieurs types de tubes, de colonnes ou de plateaux présentant plusieurs microcupules, qui sont, pour la plupart, disponibles dans le commerce.

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