Methods and materials relating to g protein-coupled...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/11 (2006.01) C07H 21/02 (2006.01) C07H 21/04 (2006.01) C12N 5/10 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2395731

The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted GPCR-like polypeptide. These polynucleotides comprise nucleic acid sequences isolated from cDNA libraries from human leukocyte mRNA (GIBCO Laboratories) (SEQ ID NO: 1), from human adult liver mRNA (GIBCO) (SEQ ID NO: 10); from human adult kidney mRNA (GIBCO) (SEQ ID NO: 17); from human adult brain mRNA (GIBCO) (SEQ ID NO: 26) and from human adult kidney mRNA (Invitrogen) (SEQ ID NO: 33). Other aspects of the invention include vectors containing processes for producing novel human secreted GPCR-like polypeptides, and antibodies specific for such polypeptides.

L'invention concerne des polynucléotides, des polypeptides codés par de tels polynucléotides, et des mutants ou des variantes de ceux-ci correspondant à un polypeptide de type récepteur couplé à une protéine G sécrété par l'organisme humain. Ces polynucléotides contiennent des séquences d'acides nucléiques isolées de bibliothèques d'ADNc provenant d'ARNm de leucocytes humain (laboratoires GIBCO) (SEQ ID NO: 1), d'ARNm de foie adulte humain (GIBCO) (SEQ ID NO: 10), d'ARNm de rein adulte humain (GIBCO) (SEQ ID NO: 17), d'ARNm de cerveau adulte humain (GIBCO) (SEQ ID NO: 26), et d'ARNm de rein adulte humain (Invitrogen) (SEQ ID NO: 33). D'autres modes de réalisation de l'invention font intervenir des vecteurs comportant des procédés de fabrication de polypeptides de type récepteurs couplés à une protéine G sécrétés par l'organisme humain, et d'anticorps spécifiques à ces polypeptides.

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