Methods and materials using signaling probes

Details Methods and materials using signaling probes Methods and materials using signaling probes

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) A01K 67/027 (2006.01) C12N 1/00 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 5/02 (2006.01) C12N 5/10 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01) C40B 40/02 (2006.01) C40B 50/06 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2556418

The present invention relates to methods of isolating cells or generating cell lines using signaling probes that produce a signal upon hybridization to a target sequence. Other methods that utilize the signaling probe include methods of quantifying the level of RNA expression, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. The invention also provides protease probes. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures are provided.

La présente invention concerne des méthodes d'isolation de cellules ou de génération de lignées cellulaires, au moyen de sondes de signalisation qui produisent un signal suite à l'hybridation d'une séquence cible. D'autres méthodes utilisant la sonde de signalisation comprennent des méthodes de quantification du niveau de l'expression d'ARN, des méthodes d'identification d'événements de recombinaison génétique dans des cellules vivantes, et des méthodes de génération d'un animal transgénique à l'aide des cellules isolées. Cette invention a aussi pour objet des sondes de protéase, ainsi que des sondes de signalisation et des sondes de protéase qui forment des structures en forme de boucle/tige, des structures de jonction à trois bras, et des structures en forme d'haltère.

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