Methods and means for exact replacement of target dna in...

Details Methods and means for exact replacement of target dna in... Methods and means for exact replacement of target dna in...

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01)

Patent

CA 2689345

Methods and means are provided for the exact exchange in eukaryotic cells, such as plant cells, of a target DNA sequence for a DNA sequence of interest through homologous recombination, whereby the selectable or screenable marker used during the homologous recombination phase for temporal selection of the gene replacement events can subsequently be removed without leaving a foot-print employing a method for the removal of a selected DNA flanked by two nucleotide sequences in direct repeats.

L'invention concerne des procédés et des moyens permettant l'échange précis dans des cellules eucaryotes, telles que des cellules végétales, d'une séquence d'ADN cible par une séquence d'ADN d'intérêt par l'intermédiaire d'une recombinaison homologue, au moyen desquels le marqueur pouvant être sélectionné ou criblé utilisé pendant la phase de recombinaison homologue pour la sélection des événements de remplacement de gènes peut être par la suite enlevé sans laisser d'empreinte en employant un procédé permettant l'enlèvement d'un ADN sélectionné flanqué de deux séquences nucléotidiques dans des unités de répétition directes.

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