Methods and means for modification of plant flowering...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C07K 14/415 (2006.01) C07K 16/16 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2384355

Provided are isolated nucleic acid molecules which comprises VRN1 nucleotide sequences, which encode a polypeptide which is capable of specifically altering the vernalisation response of a plant into which the nucleic acid is introduced and ex-pressed. Examples include cDNA and gDNA sequences (see e.g. Annex I). Also provided are variant molecules which may be derivatives or homologues (e.g. alleles, or paralogues such as RTV1), plus also complementary molecules. Corresponding polypeptides form a further part of the invention. The invention also provides methods and materials for preparing and using these molecules e.g. in the production of plants having modified vernalisation characteristics. Also methods for influencing and assessing the ver-nalisation phenotype of a plant.

L'invention concerne des molécules d'acide nucléique isolées comprenant des séquences nucléotidiques VRN1 qui codent un polypeptide capable d'altérer spécifiquement la réponse de vernalisation d'une plante dans laquelle l'acide nucléique est introduit et exprimé. Des exemples comprennent des séquences cDNA et gDNA (voir par exemple Annexe 1). L'invention concerne également des molécules variantes qui peuvent être des dérivés ou homologues (par exemple des allèles, ou des paralogues comme RTV1) ainsi que des molécules complémentaires. La présente invention porte également sur des polypeptides correspondants et sur les procédés et les matières permettant de préparer et d'utiliser ces molécules, par exemple, pour la production de plantes présentant des caractéristiques de vernalisation modifiées, ainsi que sur les procédés pour influencer et évaluer le phénotype de vernalisation d'une plante.

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