C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12M 1/36 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2647786
The present invention provides a nucleic acid sequencing method. The method comprises enriching a nucleic acid sample for target nucleic acids, where the nucleic acid sample is enriched through at least a first round of hybridization selection and amplification, and a second round of hybridization selection and amplification. The enriched nucleic acids are in a form convenient for sequencing with the Cantaloupe sequencing technology, which employs shotgun sequencing by hybridization (SBH) of immobilized rolling circle amplicons.
La présente invention concerne un procédé de séquençage d'acide nucléique. Le procédé consiste à prendre un échantillon d'acide nucléique et à produire par enrichissement des acides nucléiques cibles en deux tours de sélection-amplification par hybridation. Les acides nucléiques enrichis sont sous une forme convenant au séquençage selon la méthode Cantaloupe qui est un séquençage en aveugle par hybridation d'amplicons en cercles tournants immobilisés.
Belouchi Abdelmajid
Berube Pierre
Geoffroy Steve
Keith Tim
Linnarsson Sten
Deeth Williams Wall Llp
Genizon Biosciences Inc.
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