Methods and means for removal of a selected dna sequence

Details Methods and means for removal of a selected dna sequence Methods and means for removal of a selected dna sequence

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/52 (2006.01)

Patent

CA 2603177

A method is described for the exact removal of a selected subfragment from a DNA molecule by intrachromosomal recombination between two directly repeated DNA sequences using a rare-cleaving double stranded break inducing DNA endonuclease expressed under control of a micro-spore specific promoter. This method can be applied in a method for the exact exchange of a target DNA fragment for a DNA fragment of interest in plant cells and plants.

L'invention concerne un procédé permettant la suppression précise d'un sous-fragment choisi d'une molécule d'ADN par recombinaison intrachromosomique entre deux séquences d'ADN répétées directes, comprenant l'utilisation d'une ADN endonucléase à faible fréquence de coupure induisant une cassure double brin, dont l'expression est régulée par un promoteur spécifique des microspores. Ce procédé peut être appliqué dans un processus permettant le remplacement précis d'un fragment d'ADN cible par un fragment d'ADN choisi dans des cellules végétales et dans des plantes.

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