Methods and means for use of hla-dq restricted t-cell...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/13 (2006.01) A23L 1/29 (2006.01) A23L 1/305 (2006.01) A61K 31/7088 (2006.01) A61K 38/04 (2006.01) A61K 38/17 (2006.01) A61K 38/55 (2006.01) A61K 39/00 (2006.01) A61K 39/395 (2006.01) A61K 48/00 (2006.01) A61P 1/00 (2006.01) A61P 37/08 (2006.01) C07K 7/04 (2006.01) C07K 14/415 (2006.01) C07K 14/705 (2006.01) C07K 14/725 (2006.01) C07K 16/18 (2006.01) C07K 17/00 (2006.01) C12N 15/12 (2006.01) C12N 15/63 (2006.01) C12Q 1/02 (2006.01) G01N 33/53 (2006.01) G01N 33/564 (2006.01) G01N 33/566 (2006.01) G01N

Patent

CA 2443886

The present invention provides an isolated or recombinant HLA-DQ restricted T- cell receptor or functional equivalent and/or fragment thereof capable of recognizing a prolamine-derived peptide. The present invention also provides isolated, recombinant or synthetic prolamine-derived peptides involved in food- related immune enteropathy. In yet another embodiment the invention provides a diagnostic kit comprising an isolated or recombinant HLA-DQ restricted T-cell receptor according to the invention or host cell comprising a T-cell receptor according to the invention or an antibody according to the invention and a suitable means of detection. Such a diagnostic kit is, for example, very useful for detecting in food, food components or samples from (suspected) patients the presence of prolamine-derived peptide involved in food-related immune enteropathy (for example: celiac sprue, tropical sprue, giardiasis or food allergies of childhood).

L'invention concerne un récepteur de cellules T restreint au HLA-DQ isolé ou recombinant, ou un équivalent fonctionnel et/ou un fragment dudit élément pouvant reconnaître un peptide dérivé de prolamine. L'invention concerne également des peptides dérivés de prolamine isolés, recombinants ou synthétiques impliqués au niveau de l'entéropathie immunitaire d'origine alimentaire. Dans un autre mode de réalisation, l'invention concerne une trousse diagnostique comprenant un récepteur de cellules T restreint au HLA-DQ isolé ou recombinant correspondant à l'invention, ou une cellule hôte comprenant un récepteur de cellules T correspondant à l'invention, ou un anticorps correspondant à l'invention, et un moyen de détection approprié. Une telle trousse diagnostique est, par exemple, très utile pour détecter, dans des aliments, des composants alimentaires ou des échantillons alimentaires provenant de patients (suspectés), la présence de peptides dérivés de prolamine impliqués dans une entéropathie immunitaire d'origine alimentaire (par exemple: sprue caeliaque, sprue tropicale, lambliase ou allergies alimentaires de l'enfance).

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