Methods and oligonucleotides for the detection of salmonella...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2451498

A method for detecting a Salmonella species, E. coli O157:H7, or Listeria monocytogenes is disclosed. The method involves amplifying a genomic nucleotide sequence of a corresponding species and detecting the amplification product. Various primers and probes that can be used in the method are also disclosed. In one embodiment, the amplification step of the method is accomplished by real-time PCR and the amplification product is detected by fluorescence resonance energy tranfer using a pair of labeled polynucleotides.

L'invention concerne un procédé de détection d'une espèce Salmonella E. coli O157:H7, ou Listeria monocytogènes. Le procédé consiste à amplifier une séquence nucléotidique génomique d'une espèce correspondante et à détecter le produit d'amplification. L'invention traite également de divers amorceurs et sondes que le procédé peut utiliser. Dans un mode de réalisation, l'étape d'amplification du procédé est réalisée par PCR en temps réel, le produit d'amplification étant détecté par transfert d'énergie par résonance de fluorescence utilisant une paire de polynécléotides étiquetés.

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