Methods and organisms for the growth-coupled production of...

C - Chemistry – Metallurgy – 12 – N

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C12N 1/21 (2006.01) C12N 1/00 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 15/00 (2006.01) C12N 15/52 (2006.01) C12P 7/18 (2006.01)

Patent

CA 2695706

The invention provides a non- naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby theone or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non--naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4- butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.

L'invention concerne un micro-organisme non présent à l'état naturel comprenant une ou plusieurs ruptures de gène, la ou les ruptures de gène se produisant dans des gènes codant pour l'enzyme obligatoire au couplage de la production de 1,4-butanediol à la croissance du micro-organisme lorsque la rupture de gène réduit l'activité de l'enzyme, moyennant quoi la ou les ruptures de gène confèrent une production couplée à une croissance stable de 1,4-butanediol sur le micro-organisme non présent à l'état naturel. Le micro-organisme peut comprendre en outre un gène codant pour une enzyme dans une voie biosynthétique pour le 1,4-butanediol (BDO). L'invention concerne en outre des procédés d'utilisation de micro-organismes pour produire du BDO.

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