Methods and products for the synthesis of oligosaccharide...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/54 (2006.01) C07H 21/00 (2006.01) C07K 16/40 (2006.01) C12N 9/10 (2006.01) C12N 9/96 (2006.01) C12N 15/62 (2006.01) C12P 21/00 (2006.01) C12P 21/08 (2006.01) C12Q 1/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/567 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2075949

2075949 9112340 PCTABS00006 The invention relates to a gene and a gene product obtained by the expression of the gene where the gene is obtained by a method, comprising: (i) isolating a first cell possessing a post-translational characteristic of interest, said post-translational characteristic being the presence of a membrane-bound oligosaccharide or polysaccharide of interest on the surface of said first cell, the presence of a soluble oligosaccharide or polysaccharide of interest in an extract of said first cell, or the presence of a particularly glycosyltransferase activity in an extract of said first cell; (ii) creating a genetic library of either cDNA or genomic DNA from the genetic material of said isolated first cell; (iii) transforming host cells with said genetic library; and (iv) screening said transformed host cells for a host cell containing said post-translational characteristic, thereby obtaining a cell containing said gene.

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