Methods and reactive compositions suitable for the...

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150/14, 150/3

G01N 33/52 (2006.01) G01N 31/22 (2006.01) G01N 33/84 (2006.01)

Patent

CA 1227112

"METHODS AND REACTIVE COMPOSITIONS SUITABLE FOR THE CO LORINIETRIC DETERMINATION OF METALS" Abstract The present Patent Application refers to a formula- tion for a colorimetric reagent, suitable, among other applications, to the determination of ferric iron of serum. Such formulation consists of two aqueous solutions only, one of which, acting as chromogenic reagent, cor- sists of a buffer, a soluble salt of an alkali or alkali- earth metal, chromazurol S, a either cationic or non ionic surfactant, the other solution, acting as masking reagent, consisting of a complexing agent, and if desir- ed of the same components of the chromogenic reagent, able to prevent the formation of the complex between the chrom- azurol, the surfactant and the iron. The complexing agent is generally consisting of a polycarboxylic organic acid and a salt of it.

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