Methods and reagents for hla class i dna typing

Details Methods and reagents for hla class i dna typing Methods and reagents for hla class i dna typing

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2081582

RAN 4095/68 Abstract Primers for amplification of specific nucleic acid sequences of the second and third exon of HLA Class I genes and probes for identifying polymorphic sequences contained in the amplified DNA can be used in processes for typing homozygous or heterozygous samples from a variety of sources and for detecting allelic variants not distinguishable by serological methods. This HLA Class I DNA typing system can be used in a forward or reverse dot-blot format that is simple and rapid to perform, produces detectable signals in minutes, and can be used for tissue typing, determining individual identity, and identifying disease susceptible individuals.

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