Methods and reagents for preservation of dna in bodily fluids

Details Methods and reagents for preservation of dna in bodily fluids Methods and reagents for preservation of dna in bodily fluids

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2313654

A method and system are provided for preserving nucleic acids in a bodily fluid, such as urine, blood, blood serum, and amniotic fluid. The preservative includes an amount of a divalent metal chelator selected from ethylenediaminetetraacetic acid (EDTA), [ethylenebis(oxyethylenenitrilo)]tetraacetic acid (EGTA) and 1,2-bis(2- aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), or salts thereof in the range of from about 0.001M to 0.1M; and an amount of at least one chelator enhancing component selected from lithium chloride, guanidine, sodium salicylate, sodium perchlorate, and sodium thiocyanate in the range of from about 0.1M to 2M.

L'invention porte sur un procédé et un système de conservation des acides nucléiques de fluides corporels, tels que l'urine, le sang, le sérum sanguin, ou le liquide amniotique. Le conservateur contient une quantité d'un chélateur de métal divalent choisi parmi l'acide éthylènediaminetétraacétique (EDTA), l'acide éthylènebis(oxyéthylènenitrilo)tétraacétique (EGTA), l'acide 1,2-bis(2-aminophénoxy)éthane-N,N,N',N'-tétraacétique (BAPTA), ou leurs sels à raison de 0,001M à 0,1M, et une quantité d'au moins un composant renforçant l'action d'au moins un des chélateurs sélectionné parmi le chlorure de lithium, la guanidine, le salicylate de sodium, le perchlorate de sodium, et le thiocyanate de sodium à raison de 0,1M à 2M.

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