Methods and structures employing non-radioactive...

Details Methods and structures employing non-radioactive... Methods and structures employing non-radioactive...

C - Chemistry, Metallurgy

07

H

195/1.12, 150/8.

C07H 21/00 (2006.01) C12Q 1/68 (2006.01) G01N 33/535 (2006.01) G01N 33/552 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1309672

ABSTRACT OF THE DISCLOSURE Non-radioactive chemically-labeled polynucleotide probes, such as non-radioactive chemically-labeled, single- stranded DNA probes, are hybridized to fixed, denatured, single-stranded genetic material having a make-up com- plementary to said probes for determining the identifi- cation and/or presence of said genetic material. The non-radioactive chemical label on said probe is employed to elicit a response therefrom so as to determine the identity and/or presence of said genetic material. It is preferred that the non-radioactive chemical label attached to the probe have an enzyme attached thereto after hy- bridization, and that the presence of the enzyme attached to the chemically-labeled probe be elicited by spectro- photometric assay techniques.

445896

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