Methods and systems for adding a reagent to an analyte in a gel

Details Methods and systems for adding a reagent to an analyte in a gel Methods and systems for adding a reagent to an analyte in a gel

G - Physics

01

N

G01N 27/447 (2006.01)

Patent

CA 2612056

The present invention relates to methods and systems for adding a reagent to an analyte in a gel. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a gel to a second vessel, such as a microtitre plate. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.

La présente invention concerne des méthodes et des systèmes pour ajouter un réactif à un analyte dans un gel. L'invention fournit en outre des méthodes et des systèmes pour transférer des mélanges de réactif d'analyte liquide d'un gel vers un deuxième récipient, tel qu'une plaque de microtitrage. L'invention est utile pour manipuler des molécules biologiques telles que des acides nucléiques, hydrates de carbone, protéines et peptides. En particulier, l'invention est utile pour séparer et éluer des protéines et des peptides à partir de gels pour isoélectrofocalisation.

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