Methods and systems for evaluating health risk factors by...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2638750

Disclosed are methods for measuring the effects of environmental, physiological, or lifestyle variables on DNA damage and DNA repair activity as well as the use of measurements of DNA damage and DNA repair activity to predict increased risk for disease. Embodiments of the methods involve the use of a combination of assays to measure DNA damage and DNA repair activity in an individual and comparing these measurements to suitable controls using the selected assays for normal healthy individuals of varying ages. In other embodiments, the methods may comprise a comparison of DNA damage levels to DNA repair levels to obtain an apparent net measurement of DNA damage accumulation.

L'invention concerne des procédés permettant de mesurer les effets de variables environnementales, physiologiques ou du mode vie sur les dommages à l'ADN et sur sa réparation, ainsi que l'utilisation de ces mesures pour prédire des risques de maladies accrus. Selon des modes de réalisation, ces procédés consistent à utiliser une combinaison de dosages pour mesurer les dommages à l'ADN et sa réparation chez un individu et à comparer ces mesures à des témoins appropriés au moyen des dosages sélectionnés pour des individus normaux de différents âges et en bonne santé. Dans d'autres modes de réalisation, ces procédés peuvent consister en une comparaison des niveaux de dommages à l'ADN aux niveaux de sa réparation pour obtenir une mesure nette apparente de l'accumulation des dommages à l'ADN.

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