Methods and systems for removing autofluorescence from images

G - Physics – 01 – N

Patent

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G01N 21/64 (2006.01)

Patent

CA 2706428

Method and systems for removing any inherent autofluorescence associated with a biological material, comprising acquiring a first reference image of the biological material; acquiring a first set of one or more images of the biological material using one or more filters corresponding to one or more information dyes; applying the one or more additional dyes to the biological material and then acquiring a second set of one or more images comprising a separate image of the biological material with each of the filters corresponding to the information dyes and a second reference image of the biological material; co registering the first and second reference images, and then removing any inherent autofluorescence exhibited in the informational images acquired

L'invention concerne des procédés et des systèmes permettant d'éliminer toute autofluorescence inhérente associée à un matériau biologique. Ces systèmes et procédés consistent : à acquérir une première image de référence du matériau biologique ; à acquérir un premier ensemble d'au moins une image du matériau biologique au moyen d'au moins un filtre correspondant à au moins un colorant additionnel ; à appliquer ledit colorant au moins sur le matériau biologique puis à acquérir un deuxième ensemble d'au moins une image comprenant une image distincte du matériau biologique avec chacun des filtres correspondant aux colorants additionnels et une deuxième image de référence du matériau biologique ; à enregistrer conjointement les première et deuxième images de référence, puis à éliminer toute autofluorescence inhérente présente dans les images d'information acquises.

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