Methods, compositions and devices for growing cells

C - Chemistry – Metallurgy – 12 – N

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C12N 5/10 (2006.01) C07K 14/535 (2006.01) C07K 14/54 (2006.01) C12M 3/02 (2006.01) C12M 3/06 (2006.01) C12N 5/02 (2006.01) C12N 5/06 (2006.01) C12N 15/19 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2062741

2062741 9015877 PCTABS00003 Methods, compositions and devices are provided for the growth of hematopoietic cells in culture. Bioreactors (10) are provided where appropriate levels of nutrients and growth factors are substantially continuously maintained in the bioreactor (10) while removing undesirable metabolic products. At least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. Elements (28, 30) are provided for maintaining the stromal cells and hematopoietic cells separately, to allow for easy removal of the hematopoietic cells.

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