C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/3.2, 150/15.
C12Q 1/34 (2006.01) C12N 9/20 (2006.01) C12Q 1/44 (2006.01)
Patent
CA 1182725
Abstract of the Disclosure The present disclosure relates to methods, compositions and elements for the determination of the amount of lipase in a sample. The method com- prises the steps of: (a) contacting the sample with a reagent compo- sition comprising: i) a lipase substrate which is a glycerol triester oil having in one of its two .alpha.-ester posi- tions a long chain alkyl group having at least 8 car- bon atoms and, in its two remaining ester positions, short chain alkyl groups such that, if the long chain alkyl group is hydrolyzed, the resulting diester is water soluble; and ii) an esterase enzyme capable of catalyz- ing the hydrolysis of a water soluble glycerol di- ester to glycerol; and (b) detecting the rate at which glycerol is formed. The compositions of the present invention include the substrate and enzyme as defined. The element of the invention comprises a support having thereon the des- cribed composition. The invention is useful for determining lipase in samples which contain endo- genous glycerol, such as blood serum find other body fluids.
420200
Babb Bruce E.
Limuti Charles M.
Mauck John C.
Eastman Kodak Company
Gowling Lafleur Henderson Llp
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