Methods, compositions, and growth and differentiation...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/02 (2006.01) C12N 5/00 (2006.01) A61K 35/12 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2487858

A method of converting differentiated non-hormone producing pancreatic cells into differentiated hormone producing cells is disclosed. The method comprises two steps: first, culturing cells under conditions which convert differentiated non-hormone producing cells into stem cells; and second, culturing stem cells under conditions which provide for differentiating stem cells into hormone-producing cells. The invention defines growth and differentiation factors that are presented to the stem cells to result in their differentiation into hormone-producing cells, especially insulin- producing cells. The invention provides a new source of large quantities of hormone producing cells such as insulin-producing cells that are not currently available for therapeutic uses such as the treatment of diabetes.

L'invention concerne un procédé de transformation de cellules pancréatiques différenciées non productrices d'hormone en cellules différenciées productrices d'hormone. Ce procédé consiste d'abord à mettre en culture des cellules dans des conditions qui permettent de transformer des cellules différenciées non productrices d'hormone en cellules souches; puis à mettre en culture ces cellules souches dans des conditions qui permettent de les différencier en cellules productrices d'hormone. L'invention définit les facteurs de croissance et de différenciation qui sont mis en contact avec les cellules souches pour induire leur différenciation en cellules productrices d'hormone, particulièrement, en cellules productrices d'insuline. Par ailleurs, l'invention concerne une nouvelle source d'obtention de cellules productrices d'hormone en grande quantité, notamment les cellules productrices d'insuline, qui ne sont pas encore disponibles pour un usage thérapeutique, tel que le traitement du diabète.

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