Methods, compositions and kits for cell separation

Details Methods, compositions and kits for cell separation Methods, compositions and kits for cell separation

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 1/02 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2486616

Methods, compositions and kits for concentrating or separating cells containing target nucleic acid are disclosed, especially from mixtures containing the cells and other components such as impurities. The methods can keep a large proportion of the cells intact, allowing the cells to be employed after separation (e.g. cultured) and/or which facilitates the recovery of nucleic acid from the cells. The method employs flocculating agents, such as polyamines or cationic detergents, to form complexes with cells causing them to aggregate and so separated from other components of the mixture. Conveniently, the separation of the aggregated cells can be effected with a solid phase which is capable of binding the cells, such as magnetic beads or filters.

L'invention concerne des procédés, des compositions et des kits pour concentrer ou séparer des cellules contenant des acides nucléiques cibles, en particulier des mélanges contenant les cellules et d'autres composants tels que des impuretés. Selon lesdits procédés, une grande proportion de cellules peuvent rester intactes, ceci permettant d'utiliser les cellules après séparation (par exemple cultivées ) et/ou faciliter la récupération des acides nucléiques des cellules. Selon l'invention, on utilise des floculants, tels que des polyamines ou des détergents cationiques, pour former des complexes avec des cellules, lesdits floculants entraînant les cellules à s'agréger et à se séparer des autres composants du mélange. La séparation des cellules agrégées peut être effectuée sans inconvénients au moyen d'une phase solide capable de lier les cellules, telle que des billes magnétiques ou des filtres.

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