Methods, compositions, and kits for detection of micro rna

Details Methods, compositions, and kits for detection of micro rna Methods, compositions, and kits for detection of micro rna

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2602497

The present invention provides methods, nucleic acids, compositions, and kits for detecting microRNA (miRNA) in samples. The methods comprise ligating two oligonucleotides together in an miRNA mediated fashion, and detection of the ligation product. The methods can further comprise amplification of the ligation product, such as by PCR. The nucleic acids, compositions, and kits typically comprise some or all of the components necessary to practice the method of the invention.

La présente invention concerne des méthodes, des acides nucléiques, des compositions et des trousses permettant de détecter un micro-ARN (miARN) dans des échantillons. Les méthodes consistent à ligaturer ensemble deux oligonucléotides d'une façon induite par un micro-ARN, puis à détecter le produit de la ligature. Les procédés peuvent également consister à amplifier le produit de la ligature, par exemple par une réaction en chaîne de la polymérase (PCR). Les acides nucléiques, les compositions et les trousses comprennent d'ordinaire certains ou l'ensemble des composants nécessaires à la mise en oeuvre de la méthode de cette invention.

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