Methods, devices and systems for characterizing proteins

Details Methods, devices and systems for characterizing proteins Methods, devices and systems for characterizing proteins

G - Physics

01

N

G01N 27/26 (2006.01) C07K 1/24 (2006.01)

Patent

CA 2551350

A method of characterizing a polypeptide, comprising providing a first capillary channel having a separation buffer disposed within, wherein the separation buffer comprises a non-crosslinked polymer solutin, a buffering aten, a detergent, and a lipophilic dye. The separation buffer is provided such that, at the time of detection, the detergent concdntration in the buffer is not above the critical micelle concentration. The polypeptide is introduced into one end of the capillary channel. An electric field is applied across a length of the capillary channel, which transports polypeptides of different sizes through the polymer solution at different rates. The polypeptides is then detected as it passes a point along the length of the capillary channel.

La présente invention concerne un procédé pour caractériser un polypeptide, comprenant la mise à disposition d'un premier canal capillaire dans lequel se trouve un tampon de séparation qui comprend une solution polymère non réticulée, un agent tampon, un détergent et un colorant lipophile. Le tampon de séparation est tel que, au moment de la détection, la concentration en détergent dans le tampon est inférieure ou égale à la concentration micellaire critique. Le polypeptide est introduit dans une extrémité du canal capillaire. Un champ électrique est appliqué à une certaine longueur du canal capillaire, qui transporte des polypeptides de différentes tailles dans la solution polymère, à des vitesses différentes. Le polypeptide est alors détecté lorsqu'il passe en un certain point de la longueur du canal capillaire.

Affiliated with

Also associated with

Rating

Methods, devices and systems for characterizing proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods, devices and systems for characterizing proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods, devices and systems for characterizing proteins will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1794163