Methods, devices and systems for characterizing proteins

Details Methods, devices and systems for characterizing proteins Methods, devices and systems for characterizing proteins

G - Physics

01

N

G01N 1/38 (2006.01) B81B 1/00 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2358683

A method of characterizing a polypeptide, comprising providing a first capillary channel (104) having a separation buffer disposed within, wherein the separation buffer comprises a non-crosslinked polymer solution, a buffering agent, a detergent, and a lipophilic dye. The separation buffer is provided such that, at the time of detection, the detergent concentration in the buffer is not above the critical micelle concentration. The polypeptide is introduced into one end of the capillary channel. An electric field is applied across the polymer solution at different rates. The polypeptide is then detected as it passes a point (176) along the length of the capillary channel.

Cette invention concerne un procédé de caractérisation d'un polypeptide qui consiste à préparer un premier canal capillaire (104) dans lequel est disposé un tampon de séparation. Le tampon de séparation comprend une solution d'un polymère non réticulé, un agent tampon, un détergent et un colorant lipophile. Le tampon de séparation est conçu de manière qu'au moment de la détection, la concentration en détergent du tampon ne dépasse pas la concentration critique en micelles. Le polypeptide est introduit dans une extrémité du canal capillaire. Un champ électrique est appliqué dans la solution de polymère à des niveaux différents. Le polypeptide est alors détecté lorsqu'il passe par un point (176) sur la longueur du canal capillaire.

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