Methods for analysis of human stem cells

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/02 (2006.01) A61K 35/28 (2006.01) A61K 48/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01) C12Q 1/04 (2006.01) C12Q 1/06 (2006.01) G01N 33/50 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2173726

The present invention provides methods for precisely and reproducibly assaying hematopoietic stem cell activity in a cell population and methods of using the assay. By expansion of hematopoietic stem cells identified by means of this assay, one may produce highly enriched or clonal populations of hematopoietic stem cells. The present invention also provides methods for producing enriched or clonal populations of other hematopoietic cell types, hematopoietic cells produced thereby, and methods of using the hematopoietic cells.

La présente invention concerne une méthode d'analyse permettant une détermination précise et reproductible de l'activité de cellules-souches hématopoïétiques dans une population de cellules et l'utilisation de ce test. Par l'expansion des cellules-souches hématopoïétiques identifiées au moyen de cet essai, on peut produire des clones ou des populations enrichies en cellules-souches hématopoïétiques. La présente invention concerne également des méthodes pour produire des clones ou des populations enrichies en d'autres types de cellules hématopoïétiques, les cellules hématopoïétiques ainsi produites et l'utilisation de ces cellules hématopoïétiques.

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