Methods for antibody library screening

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 30/04 (2006.01) A61K 45/00 (2006.01) A61K 49/00 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) C40B 40/02 (2006.01) C40B 40/10 (2006.01)

Patent

CA 2580080

The present invention provides an improved method of screening a library of molecules to identify or select one or more members thereof which are candidate binding partners for one or more target entities: (a) contacting an expression library with one or more target entities; (b) subjecting said target entities to at least one washing step; (c) separating target entities which have become bound to one or more members of the expression library from unbound members of the expression library by separation through an organic phase, thereby separating candidate binding partners for said target entities from other library members.

La présente invention concerne un procédé amélioré de criblage d'une banque de molécules afin d'identifier ou de sélectionner un ou plusieurs éléments dans celle-ci qui sont des partenaires de liaison candidats pour une ou plusieurs entités cibles et consistant: (a) à mettre une banque d'expression en contact avec une ou plusieurs entités cibles ; (b) à soumettre lesdites entités cibles à au moins une étape de lavage ; (c) à séparer les entités cibles, qui se sont liées à un ou plusieurs éléments de la banque d'expression, des éléments non liés de la banque d'expression par séparation par une phase organique, séparant ainsi les partenaires de liaison candidats pour lesdites entités cibles des autres éléments de la banque.

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