Methods for antibody library screening

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 30/04 (2006.01) A61K 45/00 (2006.01) A61K 49/00 (2006.01) G01N 33/543 (2006.01) G01N 33/567 (2006.01) G01N 33/68 (2006.01) C12Q 1/68 (2006.01) C40B 40/02 (2006.01) C40B 40/10 (2006.01)

Patent

CA 2579937

The present invention provides a method of screening a library of molecules to identify and/or select one or more members thereof which are candidate binding partners for one or more ligands comprising: a) contacting an expression library in solution with one or more ligands; b) capturing ligands which have become bound to one or more members of the expression library onto a solid phase; and c) detecting the presence of a ligand, thereby detecting the presence of one or more members of the expression library which are candidate binding partners for the ligand.

L'invention concerne un procédé permettant de cribler une banque de molécules afin d'identifier et/ou de choisir un ou plusieurs membres de celle-ci qui sont des partenaires de liaison potentiels d'un ou de plusieurs ligands. Ce procédé consiste à: a) placer une banque d'expression en solution en contact avec un ou plusieurs ligands, b) capturer les ligands qui ont formé une liaison avec un ou plusieurs membres de la banque d'expression sur une phase solide, et c) détecter la présence d'un ligand, de manière à détecter ainsi la présence d'un ou de plusieurs membres de la banque d'expression qui sont des partenaires de liaison potentiels du ligand.

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